Road Safety Audits (RSAs) are a formal safety examination of an existing or future roadway or off-road facility and are conducted by an independent, experienced, multidisciplinary team. The purpose of Bicycle Road Safety Audit Guidelines and Prompt Lists is to provide transportation agencies and RSA teams with a better understanding of the safety of cyclists in the transportation system when conducting an RSA. These guidelines present the RSA team with an overview of basic principles of the safety of cyclists and potential issues affecting cyclists. They also provide information on how to conduct an RSA and effectively assess the safety of cyclists. Prompt lists describe safety considerations when conducting a cyclist-specific RSA. These guidelines will help RSA teams evaluate and suggest a multimodal approach to safety by improving the safety of cyclists and all roadway users.